Sevoflurane Limits Glioma Progression by Regulating Cell Proliferation, Apoptosis, Migration, and Invasion via miR-218-5p/DEK/β-Catenin Axis in Glioma

Sevoflurane Limits Glioma Progression by Regulating Cell Proliferation, Apoptosis, Migration, and Invasion via miR-218-5p/DEK/β-Catenin Axis in Glioma

Blog Article

Yingying Qi, Lina Guo, Yanchao Liu, Tonghang Zhao, Xianwen Liu, Yang Zhang Department of Anesthesiology, Liaocheng People’s Hospital, Liaocheng, Shandong, People’s Republic of ChinaCorrespondence: Yang ZhangDepartment of Anesthesiology, Liaocheng People’s Hospital, No.67, Dongchang West Road, Liaocheng, 252000, Shandong, People’s Republic of ChinaTel +86-0635-8276414Email [email protected]: Sevoflurane (SEV) is a frequently used volatile anesthetic in cancer surgery.Sevoflurane treatment has been shown to suppress the migration and invasion of several human cancer cells.However, the effect of sevoflurane on 5 Piece Full Sleigh Bedroom glioma remains largely unclear.

Methods: Glioma cell lines (U251 and U343) were treated by various concentrations of sevoflurane.3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), flow cytometry assay, and transwell assay were performed to detect the cell viability, apoptosis, migration and invasion.Western blot assay was employed to detect the protein levels of β-catenin, c-Myc, CyclinD1, β-catenin, N-cadherin, vimentin, and DEK.Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine the expression level of miR-218-5p.The target interaction between miR-218-5p and DEK was predicted through bioinformatics analysis and verified by dual-luciferase reporter assay system.

Results: We found that sevoflurane aberrantly inhibited the abilities on viability, migration, invasion, EMT and β-catenin signaling and promoted cell apoptosis in U251 and U343 cells in a dose-dependent manner.MiR-218-5p strikingly suppressed the abilities of proliferation, migration, invasion rather than apoptosis and activation of β-catenin signaling.Sevoflurane could facilitate the miR-218-5p expression, and its suppressing effects on glioma cells were reversed by pre-treatment with miR-218-5p inhibitors or pcDNA3.1/DEK in vitro and in vivo.Silencing of miR-218-5p reverted sh-DEK and sevoflurane-induced repression on proliferation, migration, Bus Bins invasion, and β-catenin signaling, and promotion on apoptosis in the glioma cells.

Conclusion: Our data showed that sevoflurane inhibited the proliferation, migration, invasion, and enhanced the apoptosis in glioma cells through regulating miR-218-5p/DEK/β-catenin axis.Keywords: glioma, sevoflurane, miR-218-5p, DEK, β-catenin signaling pathway.